Journal: Nature Communications

Article Title: Sialic acid exacerbates polycystic ovary syndrome in mice by modulating gut microbiota-mediated bile acid metabolism and FXR activation

doi: 10.1038/s41467-026-71365-4

Figure Lengend Snippet: A , B Ileal mRNA levels of Il−22 and Reg3β ( A ), and serum IL-22 levels ( B ) in Neu5Ac-treated mice ( n = 5 mice per group). C , D Ileal mRNA levels of Il−22 and Reg3β ( C ), and serum IL-22 levels ( D ) in L. sa -treated mice ( n = 5 mice per group). E – G Ileal mRNA levels of Il−22 and Reg3β , and serum IL-22 levels in CAPE- ( E ) and Gly-β-MCA-treated mice ( F ), and TUDCA-treated Fxr △IE mice ( G , n = 5 mice per group). H Schematic diagram illustrating the experimental design of rMuIL-22 treatment. I GTT and ITT assays ( n = 5 mice per group). GTT: ## p = 0.0046 (15 min), # p = 0.0136 (30 min), ## p = 0.0083 (60 min) and # p = 0.0372 (90 min) for rMuIL-22 + Neu5Ac + DHEA vs Neu5Ac + DHEA. ITT: ## p = 0.0044 (30 min) for rMuIL-22 + Neu5Ac + DHEA vs Neu5Ac + DHEA. J Estrous cycle assessment based on vaginal cytology. K Representative H&E-stained images (scale bar: 200 μm). L Quantification of cystic follicles and corpora lutea ( n = 5 mice per group). Data are presented as mean ± SD. One-way ANOVA followed by Tukey’s post hoc test ( A – G , J and L ), and two-way repeated-measures ANOVA with Sidak’s multiple-comparisons correction ( I ) were performed.

Article Snippet: For IL-22 treatment, mice were injected with recombinant murine IL-22 (rMuIL-22, HY-P7079, MCE) at 1 mg/kg daily for 21 days .

Techniques: Staining

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 2. The effect of PNU282987 on IL-22 production. (A) The expression level of α7nAChR in liver tissue was detected by immunohistochemistry (scale, 100 μm, 50um). (B) The integrated optical density (IOD) of α7nAChR. (C) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in liver tissue. (D and E) Densitometric analysis of protein bands in liver tissue. (F) The level of IL-22 in serum was detected by ELISA. (G) Western blot analysis was performed to quantify the levels of α7nAChR and IL-22RA1 in L-02 cells. (H and I) Densitometric analysis of protein bands in L-02 cells. (J) The level of IL-22 in cell supernatant was detected by ELISA. (K and L) qRT-PCR was used to measure the levels of α7nAChR and IL-22 in L-02 cells. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: Expressing, Immunohistochemistry, Western Blot, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 4. The effect of PNU282987 on liver apoptosis. (A) Hepatocyte apoptosis was determined by TUNEL staining.(B) Results of TUNEL staining analysis. (C) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in liver tissue. (D and E) Grayscale analysis of protein bands in liver tissue was conducted. (F) Western blot analysis was performed to quantify the levels of Bcl-2 and Bax in L-02 cells. (G and H) Grayscale analysis of protein bands in L-02 cells was conducted. (I) The level of IL-22 in cell supernatant was detected by ELISA. (ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: TUNEL Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cytokine

Article Title: The protective effect of the vagus nerve-α7nAChR-IL-22 pathway on acute liver injury.

doi: 10.1016/j.cyto.2024.156840

Figure Lengend Snippet: Fig. 5. The effects of exogenous IL-22 and IL-22BP on the regeneration and apoptosis of L-02 cells. (A) In the IL-22 group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (B,C and D) The grayscale analysis of protein bands in L-02 cells. (E) In the IL-22BP group, the levels of PCNA, Bcl-2 and Bax in L-02 cells were assessed using Western blotting. (F,G and H) The grayscale analysis of protein bands in L-02 cells. (*P < 0.05, **P < 0.01).

Article Snippet: Recombinant human IL-22 protein (Cat No. HY-P7039, MCE).

Techniques: Western Blot

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 4. Interleukin-22 receptor 1 (IL-22R1) mRNA expression in rheumatoid arthritis (RA) synovial tissues. Reverse transcription– polymerase chain reaction for IL-22R1 or -actin was performed using mRNA extracted from the synovial tissues of 4 RA patients (RA1– RA4). Hep-G2 cells were used as a positive control.

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Positive Control

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 5. Immunohistologic localization of IL-22R1 in synovial tissue from either patients with RA (A and C–J) or patients with osteoar- thritis (B). Dual-labeling immunofluorescence staining of RA synovial tissues (C–J) was done with anti–IL-22R1 (green) (C and G), anti- vimentin (red) (D), and anti-CD68 (red) (H). Staining of nuclei was done with 4,6-diamidino-2-phenylindole (blue) (E and I). Merged images are shown in F (merger of C, D, and E) and J (merger of G, H, and I). See Figure 4 for definitions. (Counterstained with hematoxylin; original magnification 200.)

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Labeling, Immunofluorescence, Staining

Journal: Arthritis and rheumatism

Article Title: Expression of interleukin-22 in rheumatoid arthritis: potential role as a proinflammatory cytokine.

doi: 10.1002/art.20965

Figure Lengend Snippet: Figure 6. Expression of IL-22 (A) and IL-22R1 (B and C) in synovial fibroblasts established from RA tissues (RASF) obtained from 4 RA patients (RA1–RA4). Reverse transcription–polymerase chain reac- tion for IL-22 (A), IL-22R1 (B), or -actin was performed using mRNA extracted from RASF. Western blotting was performed using specific antibodies against IL-22R1 (C). Peripheral blood mono- nuclear cells from normal donors, stimulated with phytohemagglutinin (PC) or without phytohemagglutinin (NC), were used as positive and negative controls, respectively (A). Hep-G2 was used as a positive control for IL-22R1 (B and C). See Figure 4 for other definitions.

Article Snippet: Rabbit anti-human IL-22R1 polyclonal antibodies were from Prosci Incorporated (Poway, CA).

Techniques: Expressing, Reverse Transcription, Western Blot, Positive Control